Can chromatin be stained with dyes?

Methyl blue and aniline blue, though acid dyes, stain the chromatin of the spermato- genetic cells of the mouse (especially of the primary spermatocytes) strongly. … It has been concluded that the dyes interact with the nucleic acids.

Does chromatin get stained with any dye?

Chromatin stains strongly with basic dyes. It is thought that the chromatin is most deeply stained when it is most condensed and inactive.

Which stain is used for staining chromatin?

Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

What are the stains used to stain chromosomes and chromatin?

Giemsa is a visible light dye that binds to DNA through intercalation and thus, is used for chromosome staining.

IT IS SURPRISING:  What are telomeres and what is their role in replication?

Are acidic dyes used for staining of chromatin?

An Investigation of the Anomalous Staining of Chromatin by the Acid Dyes, Methyl Blue and Aniline Blue. J Cell Sci (1962) s3-103 (64): 519–530. Methyl blue and aniline blue, though acid dyes, stain the chromatin of the spermatogenetic cells of the mouse (especially of the primary spermatocytes) strongly.

Which one of the following dyes Cannot be used for staining chromatin material?

A. Acetocarmine. The key proteins in chromatin are proteins called histones. …

What is light colored chromatin called?

Euchromatin is a lightly packed form of chromatin (DNA, RNA, and protein) that is enriched in genes, and is often (but not always) under active transcription. Euchromatin stands in contrast to heterochromatin, which is tightly packed and less accessible for transcription. 92% of the human genome is euchromatic.

What are the stains used to stain chromosomes and chromatin Class 11?

Carmine The basic dye, is used to stain nucleic acid and chromosomes, whichpossess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.

Is chromatin acidic or basic?

Histones or Basic Proteins: Chromatin of all eukaryotic cells is a nucleoprotein complex in which DNA strand is associated with proteins. The associated proteins are of two kinds; basic proteins (histones) and acidic proteins (non-histones).

Which stain gives violet or purple to chromosome?

Feulgen dye is the aldehyde specific reactive dye that stains when a reaction of aldehydes occurs with the fuchsin-sulphuric acid. It gives a characteristic colour of purple.

What stain is used to colour chromosomes?

Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.

IT IS SURPRISING:  What is a good job for someone with high functioning autism?

Which stain is used in staining the material?

Gram. Gram staining is used to determine gram status to classifying bacteria broadly based on the composition of their cell wall. Gram staining uses crystal violet to stain cell walls, iodine (as a mordant), and a fuchsin or safranin counterstain to (mark all bacteria).

Which stain is used in T banding?

Requirements for T banding:

Microscope, coverslip and slides, PBS solution, Giemsa stain, Hoechst 33258, UV lamp and acridine orange dye.

Which dye could be used in acid staining?

The three major types of dyes used in biological staining are acid dyes, basic dyes, and neutral dyes. Basic dyes stain basophilic structures (e.g. nuclei, ribosomes and GAGs). Acid dyes stain acidophilic structures (e.g. cytoplasm, basic tissue proteins). Examples of acid dyes are Indian ink, congo red, nigrosoine.

Is nucleus acidic or basic?

Nucleus is acidic in nature.

Which of the following dyes are useful to stain nuclear material?

As nucleic acid is acidic and the nucleoprotein is basic in nature, both acidic and basic dyes can be used to stain a nucleus of a cell. Complete answer: Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells.