The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required. To stain chromosomes first acetocarmine dye is prepared using the carmine. Carmine is a basic dye which is obtained from the offspring insect.
Which dye is not used to stain chromosomes?
(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.
Can chromatin be stained with dyes?
Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.
What is Acetocarmine stain?
Definition of acetocarmine
: a saturated solution of carmine in 45 percent acetic acid used especially for the rapid staining of fresh unfixed chromosomes.
Which dye is used to stain mitochondria?
The Janus green is a basic dye which is also used as a vital stain in histology. This dye is used for staining the mitochondria.
Which stain is usually used to Colour chromosomes Class 11?
Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.
What are the stains used to stain chromosomes and chromatin?
Giemsa is a visible light dye that binds to DNA through intercalation and thus, is used for chromosome staining.
What are the stains used to stain chromosomes and chromatin Class 11?
Carmine The basic dye, is used to stain nucleic acid and chromosomes, whichpossess negative charge on them. It gives chromosomes a pink colour thus, differentiating from other cellular organelles.
Which stain gives violet or purple to chromosome?
Feulgen dye is the aldehyde specific reactive dye that stains when a reaction of aldehydes occurs with the fuchsin-sulphuric acid. It gives a characteristic colour of purple.
Is Acetocarmine a basic dye?
What is Acetocarmine. Carmine is a basic dye prepared from an insect known as Coccus cacti. Acetocarmine is produced by mixing carmine with glacial acetic acid. It is a DNA-specific stain used for the visualization of super-coiled chromosomes during the different stages of mitosis.
Why is Acetocarmine used to stain chromosomes?
The stains: 2% acetocarmine and orcein are chromatin-specific dyes. They bind permanently to the nucleoprotein component of chromatin. Thats why chromatin can be visualized by treating it with the tw dyes. The dyes are very active and small quantities of them can be very efective to observe chromatine material.
What is chromosome staining?
Staining is responsible for the alternating dark and light bands on the chromosomes noted in Figure 11.1B. The most routinely used technique stains the metaphase chromosomes with Giemsa (after using the enzyme trypsin to digest proteins). Each chromosome pair stains with its own characteristic banding pattern.
Which dye we can use to stain the cell?
Malachite green – a blue-green counterstain to safranin in Gimenez staining for bacteria. This stain can also be used to stain spores. Methylene blue – stains animal cells to make nuclei more visible. Neutral/Toluylene red – stains nuclei red and may be used on living cells.
What does Sudan black stain?
Black Sudan B is used for the staining of a wide variety of lipids such as phospholipids, steroles and neutral triglycerides. Black Sudan B is not lipid specific like other Sudan dyes and can also be used for chromosome staining, Golgi apparatus and leukocyte granules.
Why is Janus Green used to stain mitochondria?
It is a basic cationic vital dye that is used to stain mitochondriain living cells without killing them. Janus Green B is chemically diethyl safranin-azo-dimethyl aniline. … The cytochrome c oxidase helps to maintain the dye in an oxidised state in the mitochondria. In its oxidized state it is bluish green in colour.