Which is the best dye for staining chromosome?

Which dye is best for staining chromosomes?

Complete answer: The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required. To stain chromosomes first acetocarmine dye is prepared using the carmine.

Which type of stains are best suitable for staining nucleus and chromosomes?

Giemsa stain is one of the best known histological stains, coloring the nuclei dark blue and the cytoplasm blue to pink, according to the acidity of the cytoplasmic contents.

Which stain is used to observe chromosomes?

Chromosomes are visualized using Giemsa staining (G-banding). Light bands represent early replicating regions, rich in guanine and cytosine nucleotides. Dark bands represent late replicating regions, rich in adenine and thymine nucleotides.

Which is best suited staining for karyotype analysis chromosomes?

Today, most karyotypes are stained with Giemsa dye, which offers better resolution of individual bands, produces a more stable preparation, and can be analyzed with ordinary bright-field microscopy.

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Which dye is not used to stain chromosomes?

(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.

Which stain is usually used to Colour chromosomes Class 11?

Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.

What is Acetocarmine stain?

Definition of acetocarmine

: a saturated solution of carmine in 45 percent acetic acid used especially for the rapid staining of fresh unfixed chromosomes.

Which one of the following is a basic stain?

Basic stains, such as methylene blue, Gram safranin, or Gram crystal violet are useful for staining most bacteria. These stains will readily give up a hydroxide ion or accept a hydrogen ion, which leaves the stain positively charged.

Which of the following is good for staining protein?

The most common method of in-gel protein detection is staining with Coomassie dye. These stains either use the G-250 (“colloidal”) or the R-250 form of the dye. Colloidal Coomassie stains can be formulated to effectively stain proteins within 1 hour and requires only water (no methanol or acetic acid) for destaining.

How do you stain chromosomes?

Q-Banding – Chromosomes are stained with fluorescent dyes, quinacrine or quinacrine mustard. Q-Band staining is similar to G-banding in that the fluorescent regions represent the AT-rich regions of the chromosome. R-Banding – Results from heat treatment in a phosphate buffer followed by staining with Giesma dyes.

Why are karyotypes stained?

G-banding, G banding or Giemsa banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. It is useful for identifying genetic diseases through the photographic representation of the entire chromosome complement.

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What is Safranin stain?

Safranin (also Safranin O or basic red 2) is a biological stain used in histology and cytology. … This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage, mucin and mast cell granules.

What are karyotypes used for?

Karyotype is a test to identify and evaluate the size, shape, and number of chromosomes in a sample of body cells. Extra or missing chromosomes, or abnormal positions of chromosome pieces, can cause problems with a person’s growth, development, and body functions.

Why Giemsa stain is used for chromosome observation?

It can identify chromosomal aberrations such as translocations and rearrangements. It stains the trophozoite Trichomonas vaginalis, which presents with greenish discharge and motile cells on wet prep. Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain.

Which chromosome regions stain darkly and which stain lightly?

Regions in the chromosome that stain rather lightly with G-banding tend to be more transcriptionally active, euchromatic, and rich with guanine and cytosine. In contrast, the chromosomal regions that stain darkly tend to be less transcriptionally active, heterochromatic, and rich with adenine and thymine.