Which of the following dyes is best suited for staining chromosomes?

Which dye is best for staining chromosomes?

Complete answer: The stain used for dying the chromosome is acetocarmine. This stain is a DNA-specific stain and is used when the study of different mitotic stages is required. To stain chromosomes first acetocarmine dye is prepared using the carmine.

Which type of stains are best suitable for staining nucleus and chromosomes?

Giemsa stain is one of the best known histological stains, coloring the nuclei dark blue and the cytoplasm blue to pink, according to the acidity of the cytoplasmic contents.

Which stain is used to observe chromosomes?

Chromosomes are visualized using Giemsa staining (G-banding). Light bands represent early replicating regions, rich in guanine and cytosine nucleotides. Dark bands represent late replicating regions, rich in adenine and thymine nucleotides.

Which is best suited staining for karyotype analysis chromosomes?

Today, most karyotypes are stained with Giemsa dye, which offers better resolution of individual bands, produces a more stable preparation, and can be analyzed with ordinary bright-field microscopy.

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Which dye is not used to stain chromosomes?

(b) Saffanin stain is not used for staining chromosomes while Basic Fuchsin, Methylene green and Carmine are used for staining chromosomes.

Which of the following dyes are useful to stain nuclear material?

As nucleic acid is acidic and the nucleoprotein is basic in nature, both acidic and basic dyes can be used to stain a nucleus of a cell. Complete answer: Although there are various nuclear stains, Methylene blue is the most commonly used nuclear stain to observe the nucleus in the cheek cells.

Which stain is usually used to Colour chromosomes Class 11?

Gentian violet, Safranin and Giemsa stain are commonly used to colour chromosomes.

Which of the following is a nuclear stain answer?

Haematoxylin (hematoxylin in North America) is a nuclear stain. Used with a mordant, haematoxylin stains nuclei blue-violet or brown.

Which of the following is good for staining protein?

The most common method of in-gel protein detection is staining with Coomassie dye. These stains either use the G-250 (“colloidal”) or the R-250 form of the dye. Colloidal Coomassie stains can be formulated to effectively stain proteins within 1 hour and requires only water (no methanol or acetic acid) for destaining.

How do you stain chromosomes?

Q-Banding – Chromosomes are stained with fluorescent dyes, quinacrine or quinacrine mustard. Q-Band staining is similar to G-banding in that the fluorescent regions represent the AT-rich regions of the chromosome. R-Banding – Results from heat treatment in a phosphate buffer followed by staining with Giesma dyes.

Is Acetocarmine a basic dye?

What is Acetocarmine. Carmine is a basic dye prepared from an insect known as Coccus cacti. Acetocarmine is produced by mixing carmine with glacial acetic acid. It is a DNA-specific stain used for the visualization of super-coiled chromosomes during the different stages of mitosis.

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When chromosomes are stained with dyes the euchromatin region found is?

When chromosomes are stained with acetocarmine, then two types of regions can be observed: Heterochromatin region: This region gets dark stain during interphase. This is genetically inactive and highly condensed region with tightly packed DNA. Euchromatin region: This region gets light stain during interphase.

Which chromosome regions stain darkly and which stain lightly?

Regions in the chromosome that stain rather lightly with G-banding tend to be more transcriptionally active, euchromatic, and rich with guanine and cytosine. In contrast, the chromosomal regions that stain darkly tend to be less transcriptionally active, heterochromatic, and rich with adenine and thymine.

Why Giemsa stain is used for chromosome observation?

It can identify chromosomal aberrations such as translocations and rearrangements. It stains the trophozoite Trichomonas vaginalis, which presents with greenish discharge and motile cells on wet prep. Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain.